Fig. 2

Hepatic GSTM1 loss aggravates alcohol-induced hepatic steatosis and liver injury. Hepatocyte-specific GSTM1 knockdown mice were generated by injected in the caudal vein injection with recombinant adenoassociated viral (AAV) 8 gene transfer vectors bearing a liver-specific promoter combination (TBG) with mouse GSTM1 shRNA sequence. Mice injected with null vector are served as control. A, B GSTM1 mRNA and protein expression and C GST activity in the hepatocyte-specific GSTM1 knockdown mice. D, E Plasma ALT and AST activities. F H&E staining. G Liver TG content. H Oil red O staining. Protein bands intensity was quantified by ImageJ. Data are presented as means ± SD. Statistical comparisons were made using one-way ANOVA with Tukey’s post hoc test or Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding control. PF pair-fed; AF alcohol-fed. A–H (n = 6)