Fig. 5

Hepatic GSTM1 loss blocks miR-743a-3p knockdown-protected ALD. Hepatocyte-specific miR-743a-3p-GSTM1 double knockdown mice were established. Mice injected with null vector are served as control. A, B Plasma ALT and AST activities. C H&E staining. D Liver TG content. E Oil red O staining. F Liver lipid metabolism genes expression. Protein band intensity was quantified by ImageJ. Data are presented as means ± SD. Statistical comparisons were made using one-way ANOVA with Tukey’s post hoc test or Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding control. PF pair-fed, AF alcohol-fed. A–F (n = 6)