Fig. 2

Macrophage depletion alleviates LPS-induced ALI in obese mice. Macrophages in lung tissue of obese mice were depleted through intratracheal instillation of clodronate liposomes (CLs). A Immunofluorescent labeling of macrophages. Macrophages are labeled in red (F4/80) and nuclei in blue (DAPI). F4/80-positive cells were counted using FLOWJO software. Scale bar, 50 µm. B, C RT-qPCR assessment of mRNA levels of M1 macrophage markers (iNOS and CD86) and M2 macrophage markers (Arg-1 and CD206) in lung tissues. D H&E staining illustrating the extent of lung pathological damage and corresponding lung injury scores. E LDH activity in lung tissue was assessed using an LDH activity assay kit. F Wet-to-dry weight ratio of the lungs. G Protein concentration in the BALF supernatant was determined using the BCA protein quantitation kit. Data are expressed as the mean ± SD, *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001