Fig. 4

Increased m6A demethylation during obesity mediates the downregulation of miR-192 expression. A, B RT-qPCR assessment of pre-miR-192 and pri-miR-192 expression in mouse lung tissues and AMs. C RT-qPCR assessment of pre-miR-192 and pri-miR-192 expression in PA-treated MH-S cells. D m6A levels comparison in lung tissues of lean vs. obese mice. E m6A RIP assessment of m6A-modified pri-miR-192 expression in lung tissues. F RT-qPCR assessment of mRNA levels of METTL3, METTL14, WTAP, FTO, ALKBH5, YTHDF1, and YTHDC2. G Western blot assessment of protein expression of METTL3, FTO, ALKBH5, and YTHDC2 in lung tissues. H Immunofluorescence of FTO (red) in freshly extracted AMs with nuclei labeled in blue (DAPI). Scale bar, 20 µm. I Western blot assessment of FTO protein expression in MH-S cells after PA or/and LPS treatment. J RT-qPCR and western blot assessment of FTO mRNA and protein expression in MH-S cells after Lv-FTO transfection. K RT-qPCR assessment of miR-192, pre-miR-192 and pri-miR-192 expression in MH-S cells after Lv-FTO transfection. L m6A RIP assessment of m6A-modified pri-miR-192 expression in MH-S cells after Lv-FTO transfection. Data are expressed as the mean ± SD, *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001