Fig. 5

miR-192 inhibition intensifies LPS-induced activation and inflammation in MH-S cells. MH-S cells were treated with the miR-192 inhibitor (Antagomir) or its control (AntagNC). A RT-qPCR of miR-192 expression post Antagomir treatment in MH-S cells. B–D ELISA measurements of protein levels of TNF-α, IL-1β, and IL-6 in the cell culture supernatant. E Flow cytometry analysis of the proportions of M1 macrophages (CD86⁺CD206⁻) and M2 macrophages (CD86⁻CD206⁺) in MH-S cells. F WB assessment of p-AKT, AKT, p-p65, and p65 levels. G Immunofluorescence of nuclear p-p65 translocation. Red for p-p65, blue for DAPI-stained nuclei. Scale bar, 20 μm. Data are expressed as the mean ± SD, **P < 0.01. ***P < 0.001. ****P < 0.0001