Fig. 7

miR-192 targets BIG1 in MH-S cells. A Bioinformatics prediction of the binding site between miR-192 and ARFGEF1 (BIG1) 3ʹ UTR, validated by dual-luciferase reporter assay. B, C RT-qPCR and western blot assessment of BIG1 mRNA and protein expression in MH-S cells after Agomir transfection. D, E RT-qPCR and western blot assessment of BIG1 mRNA and protein expression in MH-S cells after Lv-BIG1 transfection. F Flow cytometry analysis of the proportions of M1 macrophages (CD86⁺CD206⁻) and M2 macrophages (CD86⁻CD206⁺) in MH-S cells. G ELISA measurements of protein levels of TNF-α, IL-1β, and IL-6 in the cell culture supernatant. H WB assessment of p- AKT, AKT, p-p65, and p65 levels. I Immunofluorescence of nuclear p-p65 translocation. Red for p-p65, blue for DAPI-stained nuclei. Scale bar, 20 μm. Data are expressed as the mean ± SD, *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.0001