Fig. 8

Overexpression of miR-192 alleviates LPS-induced alveolar macrophage activation and inflammation. ALI modeling was performed after intratracheal instillation of miR-192 agomir in obese mice. A, B RT-qPCR detection of miR-192 expression in mouse lung tissue and alveolar macrophages. C Immunofluorescent labeling of macrophages. Macrophages are labeled in red (F4/80) and nuclei in blue (DAPI). F4/80-positive cells were counted using FLOWJO software. Scale bar, 50 µm. D, E RT-qPCR assessment of mRNA levels of M1 macrophage markers (iNOS and CD86) and M2 macrophage markers (Arg-1 and CD206) in lung tissues. F ELISA measurements of protein levels of TNF-α, IL-1β, and IL-6 in lung tissues. G RT-qPCR and western blot assessment of BIG1 mRNA and protein expression in mouse lung tissue. H RT-qPCR detection of BIG1 mRNA expression in alveolar macrophages. I WB assessment of p- AKT, AKT, p-p65, and p65 levels. J, K H&E staining illustrating the extent of lung pathological damage and corresponding lung injury scores. L LDH activity in lung tissue was assessed using an LDH activity assay kit. M Wet-to-dry weight ratio of the lungs. N Protein concentration in the BALF supernatant was determined using the BCA protein quantitation kit. Data are expressed as the mean ± SD, **P < 0.01. ***P < 0.001. ****P < 0.0001