Fig. 4

Induction of adipocyte differentiation and the expression of adiponectin by mifepristone alone in 3T3-L1 cells. A Representative fluorescent microscopic images of Bodipy 493/503 (green) fluorescence and summary (n = 3) of the concentration-dependent effects of mifepristone on the lipid accumulation on day 12. The cells were treated with mifepristone for the first 5 days, and then cultured in its absence until day 12. Scale bar, 300 µm. B Representative immunoblot (IB) images and summary (n = 3) of the concentration-dependent effects of treatment with mifepristone on the adiponectin protein expression on day 6. The expression levels of adiponectin were normalized to those of cyclophilin B, and then expressed as the fold increase from the value obtained without treatment. All data represent the mean ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 vs. the values obtained without treatment