Fig. 5

Effects of knockdown of PPARγ on the expression of adiponectin and Fabp4 during mifepristone-induced adipocyte differentiation in 3T3-L1 cells. A Summary of the qRT-PCR (n = 3) and immunoblot (IB) (n = 4) analyses of the efficacy of knockdown of PPARγ on 2 and 7 days after transfection of siRNA (10 nM). Representative IB images are also shown. The levels of PPARγ mRNA and protein were normalized by those of 18S rRNA and Rac1, respectively, and the values obtained with control siRNA were assigned a value of 1. B Representative immunoblot (IB) images and summary (n = 3) of the effects of PPARγ knockdown on the mifepristone-induced expression of adiponectin and Fabp4 proteins on day 5 after the induction of adipogenesis (i.e., 7 days after transfection of PPARγ or control siRNA). The levels of adiponectin and the Fabp4 protein expression were normalized to those of Rac1, and the values obtained with mifepristone and control siRNA were assigned a value of 1. All data represent the mean ± S.E.M. *P < 0.05; **P < 0.01