Fig. 1
From: Escaping from CRISPR–Cas-mediated knockout: the facts, mechanisms, and applications
Misinterpretation of BUB1 function owing to knockout escaping. A The wild-type cell shows strong SAC when activated. B The Bub1 knockout cell generated via frame shifting induced by CRISPR–Cas produces residual Bub1 protein, which fully supports SAC activity. C The complete Bub1 knockout cell, like through physical removal of the DNA fragment of BUB1 by generating two double-strand breaks flanking the gene, could only maintain low SAC activity