Fig. 2

Down-regulation of cGAS–STING signaling pathway reduces the activation of NLRP3 inflammasome and pyroptosis. A–C Wild type, cGAS ^−/− and sting ^−/− mice were injected with CAE and LPS (n = 6 per group). Western blot of NLRP3, ASC, cGAS, and STING in lung tissue (A ). Protein expression level of A (B). Flow cytometry was used to detect the pyroptosis of alveolar macrophages ( C). D–G Ana-1 cells were transfected with siRNA (si-cGAS/si-sting) for 48 h. WB and protein expression level of cGAS )D, E). WB and protein expression level of STING (F, G). H–K A total of 48 h after siRNA transfection, Ana-1 cells were stimulated with LPS and nigericin sodium salt. WB of cGAS, STING, P-STING and NLRP3 (H). Pyroptosis was detected by flow cytometry (I). WB of STING, P-STING and NLRP3 (J). Pyroptosis was detected by flow cytometry (K). ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001