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Fig.3 | Cellular & Molecular Biology Letters

Fig.3

From: Mitochondrial (mt)DNA–cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes (STING) signaling promotes pyroptosis of macrophages via interferon regulatory factor (IRF)7/IRF3 activation to aggravate lung injury during severe acute pancreatitis

Fig.3

Mitochondrial DNA induces pyroptosis by activating cGAS signaling. A Copy number of mitochondrial DNA and nuclear DNA in cytoplasm. B Cell fluorescence showing mitochondrial potential, red fluorescence for Mito-Tracker, and blue fluorescence for Hoechst. C, D Ana-1 cells treated with 100 ng/ml Ethbr for 72 h. Quantification of mitochondrial DNA and nuclear DNA (C). DNA and mitochondria were visualized by confocal microscopy, white arrows suggest mitochondrial DNA free from the nucleus (D). E–H A total of 72 h after application of EthBr, LPS, and nigericin sodium salt were administered to induce pyroptosis in Ana-1 cells. Mitochondrial morphology under electron microscope (E). The white arrows refer to mitochondria. WB of cGAS and NLRP3 (F). G mRNA expression levels of cGAS, STING, NLRP3, caspase-1, IL-18, and IL-1β. H Pyroptosis of Ana-1 cells was detected by flow cytometry. ns, no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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