Fig. 5

Downregulation of STING reduced IRF7 and P-IRF3 to improve pyroptosis. A Wild type mice and sting ^−/− knockout mice were used to establish SAP animal models in the SAP group and the sting ^−/− group, respectively. The CON group was injected with normal saline. WB of IRF7, IRF3 and P-IRF3 in alveolar macrophages. B–D WB of IRF7, IRF3, P-IRF3, cytoplasmic P-IRF3, nuclear P-IRF3. E The expression and distribution of P-IRF3 were observed under fluorescence microscope. F–H Ana-1 cells were transfected with siRNA (si-IRF3/si-IRF7) for 48 h. F, G WB and protein expression level of IRF3. H, I WB and protein expression level of IRF7. J, K A total of 48 h after siRNA transfection, LPS and nigericin sodium salt were used to induce pyroptosis in Ana-1 cells. Pyroptosis was detected by flow cytometry. **p < 0.01, ***p < 0.001, ****p < 0.0001