Fig. 6

DNMT3A acted as a target gene of miR-29b-3p in CVB3-induced myocarditis and reduced pyroptosis. A Bioinformatic analysis predicted the potential targets of miR-29b-3p. B The seed region of miR-29b-3p matches the sequence (nucleotides 825–831) on 3′ UTR of DNMT3A. C, D Total RNA and protein were extracted from heart tissues to detect the expression levels of DNMT3A. E, F The primary cardiomyocytes were transiently transfected with 20 μM miR-29b-3p inhibitor or mimic. A total of 48 h later, total protein was extracted and subjected to western blotting. The relative expression of DNMT3A was represented as a histogram and normalized using GAPDH. G, H Primary cardiomyocytes were transfected with 2.5 μg plasmid (phage-DNMT3A or phage-vector) for 48 h, and then total RNA and protein were extracted to evaluate DNMT3A expression. I CVB3-infected cardiomyocytes were transfected with 2.5 μg plasmid as indicated. After 48 h, pyroptosis level was evaluated by western blotting. N = 3, ####p < 0.0001,** p < 0.01, ***p < 0.001, and ****p < 0.0001