Open Access

Identification of microsatellite markers in the rye genome

  • Hanna Bolibok1,
  • Monika Rakoczy-Trojanowska1Email author,
  • Małgorzata Wyrzykowska1,
  • Magdalena Radecka2 and
  • Wacław Orczyk2
Cellular & Molecular Biology LettersAn International Journal200611:23

DOI: 10.2478/s11658-006-0023-5

Received: 25 January 2005

Accepted: 18 April 2006

Published: 01 June 2006

Abstract

The rye genomic library, which consists of DNA fragments in the range of 0.5–1.1 kb, was screened for the presence of tri-and tetranucleotide and compound microsatellites. Of the 1,600,000 clones analysed, 102 clones were positive and 41 were suitable for SSR primer pair design. Twenty-six primer pairs amplified specific products, and six of them were capable of detecting polymorphism among 30 rye accessions of different genetic backgrounds. Using a set of Chinese Spring-Imperial wheat-rye addition lines, it was possible to locate 3 newly identified microsatellites on chromosomes 3R, 4R and 7R.

Key words

Rye Genomic library SSR Microsatellite Wheat-rye addition lines

Advertisement