Open Access

Genotyping of recombinant Pichia pastoris strains

  • José M. Viader-Salvadó1Email author,
  • Eddy L. Cab-Barrera1,
  • Luis J. Galán-Wong1 and
  • Martha Guerrero-Olazarán1
Cellular & Molecular Biology LettersAn International Journal11:29

DOI: 10.2478/s11658-006-0029-z

Received: 23 November 2005

Accepted: 15 May 2006

Abstract

A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production.

Key words

Pichia pastoris Amplified-fragment length polymorphism (AFLP) Clonal variation

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