Open Access

The inhibition of in vivo tumorigenesis of osteosarcoma (OS)-732 cells by antisense human osteopontin RNA

  • Si-Jin Liu1, 6Email author,
  • Dao-Qiang Zhang2,
  • Xiu-Mei Sui2,
  • Lin Zhang3,
  • Zi-Wei Cai4,
  • Li-Qiu Sun4,
  • Ya-Jun Liu5,
  • Yan Xue5 and
  • Guo-Fa Hu1
Cellular & Molecular Biology LettersAn International Journal200713:31

DOI: 10.2478/s11658-007-0031-0

Received: 11 April 2007

Accepted: 22 May 2007

Published: 19 October 2007

Abstract

Osteopontin (OPN) is a secreted, non-collagenous, sialic acid-rich protein which functions by mediating cell-matrix interactions and cellular signaling via binding with integrins and CD44 receptors. An increasing number of studies have shown that OPN plays an important role in controlling cancer progression and metastasis. OPN was found to be expressed in many human cancer types, and in some cases, its over-expression was shown to be directly associated with poor patient prognosis. In vitro cancer cell line and animal model studies have clearly indicated that OPN can function in regulating the cell signaling that ultimately controls the oncogenic potential of various cancers. Previous studies in our laboratory demonstrated that OPN is highly expressed in human osteosarcoma (OS) cell line OS-732. In this study, we successfully reduced the tumorigenecity of OS-732 cells xenotransplanted into nude mice, using the antisense human OPN (hOPN) RNA expression vector.

Key words

Osteopontin Osteosarcoma Antisense RNA

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