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Syntaxin 8 has two functionally distinct di-leucine-based motifs

Cellular & Molecular Biology Letters volume 13, pages 144–154 (2008)Cite this article

Abstract

Syntaxin 8 has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These have been shown to function as the machinery for the homotypic fusion of late endosomes. Recently, we showed that syntaxins 7 and 8 cycle through the plasma membrane, and that the di-leucine-based motifs in the cytoplasmic domain of syntaxins 7 and 8 respectively function in their endocytic and exocytic processes. However, we could not elucidate the mechanism by which syntaxin 8 cycles through the plasma membrane. In this study, we constructed several different syntaxin 8 molecules by mutating putative di-leucine-based motifs, and analyzed their intracellular localization and trafficking. We found a di-leucine-based motif in the cytoplasmic domain of syntaxin 8. It is similar to that of syntaxin 7, and functions in its endocytosis. These results suggest that in the cytoplasmic domain, syntaxin 8 has two functionally distinct di-leucine-based motifs that act independently in its endocytic and exocytic processes. This is the first report on two di-leucine-based motifs in the same molecule acting independently in distinct transport pathways.

Abbreviations

HA:

hemagglutinin

NSF:

N-ethylmaleimide-sensitive factor

SNAP:

soluble NSF-attachment protein

SNARE:

SNAP-receptor

TGN:

trans-Golgi network

t-SNARE:

target-SNARE

v-SNARE:

vesicle-SNARE

vti1b:

Vps10p tail interactor 1b

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Authors and Affiliations

  1. Department of Cell Physiology, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan

    Kazuo Kasai, Kei Suga & Kimio Akagawa

  2. Toyama Chemical Co., Ltd., Shinjuku, Tokyo, 160-0023, Japan

    Kazuo Kasai

  3. Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan

    Kazuo Kasai & Tetsuro Izumi

Authors
  1. Kazuo Kasai
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  2. Kei Suga
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  3. Tetsuro Izumi
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  4. Kimio Akagawa
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Correspondence to Kimio Akagawa.

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Kasai, K., Suga, K., Izumi, T. et al. Syntaxin 8 has two functionally distinct di-leucine-based motifs. Cell Mol Biol Lett 13, 144–154 (2008). https://doi.org/10.2478/s11658-007-0043-9

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  • DOI: https://doi.org/10.2478/s11658-007-0043-9

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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