Open Access

The induction of apoptosis by daunorubicin and idarubicin in human trisomic and diabetic fibroblasts

  • Sylwia Dragojew1,
  • Agnieszka Marczak1,
  • Janusz Maszewski2,
  • Krzysztof Ilnicki3 and
  • Zofia Jóźwiak1Email author
Cellular & Molecular Biology LettersAn International Journal200813:45

DOI: 10.2478/s11658-007-0045-7

Received: 3 April 2007

Accepted: 27 August 2007

Published: 10 April 2008

Abstract

In this study, we investigated apoptosis induced in human trisomic and diabetic fibroblasts by daunorubicin (DNR) and its derivative, idarubicin (IDA). The cells were incubated with DNR or IDA for 2 h and then cultured in a drug-free medium for a further 2–48 h. The apoptosis in the cultured cell lines was assessed by biochemical analysis. We found that both drugs induced a timedependent loss of mitochondrial membrane potential, and a significant increase in intracellular calcium and caspase-3 activity. Mitochondrial polarization and changes in the level of intracellular calcium were observed during the first 2–6 h after drug treatment. Caspase-3 activation occurred in the late stages of the apoptotic pathway. Our findings also demonstrated that idarubicin was more cytotoxic and more effective than daunorubicin in inducing apoptosis in trisomic and diabetic fibroblasts.

Key words

Daunorubicin Idarubicin Apoptosis Fibroblasts Down’s syndrome Diabetes

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