Open Access

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a “density cushion”

  • Anna Wilk1,
  • Katarzyna Urbańska1,
  • David E. Woolley2 and
  • Włodzimierz Korohoda1Email author
Cellular & Molecular Biology LettersAn International Journal200813:7

DOI: 10.2478/s11658-008-0007-8

Received: 14 June 2007

Accepted: 22 November 2007

Published: 29 February 2008

Abstract

This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a “density cushion”. The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The “density cushion” method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.

Keywords

Cell electrophoresis Cell separation Cell surface

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