Skip to main content
Download PDF
  • Research Article
  • Published:

The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

Cellular & Molecular Biology Letters volume 13, pages 585–598 (2008)Cite this article

Abstract

We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.

Abbreviations

CME:

coefficient of movement efficiency

PBS:

phosphate buffered saline

TLC:

thin-layer chromatography

TRITC phalloidin:

phalloidin-conjugated tetramethylrhodamine B isothiocyanate

References

  1. Xiao, K., Yi, Y.H., Wang, Z.Z., Tang, H.F., Li, Y.Q. and Lin, H.W. A cytotoxic triterpene saponin from the root bark of Aralia dasyphylla. J. Nat. Prod. 62 (1999) 1030–1032.

    Article  PubMed  CAS  Google Scholar 

  2. Barthomeuf, C., Debiton, E., Mshvildadze, V., Kemertelidze, E. and Balansard, G. In vitro activity of hederacolchisid A1 compared with other saponins from Hedera colchica against proliferation of human carcinoma and melanoma cells. Planta Med. 68 (2002) 672–675.

    Article  PubMed  CAS  Google Scholar 

  3. Marquina, S., Maldonado, N., Garduno-Ramirez, M.L., Aranda, E., Villarreal, M.L., Navarro, V., Bye, R., Delgado, G. and Alvarez, L. Bioactive oleanolic acid saponins and other constituents from the roots of Viguiera decurrens. Phytochemistry 56 (2001) 93–97.

    Article  PubMed  CAS  Google Scholar 

  4. Podolak, I., Galanty, A., Trojanowska, D. and Janeczko, Z. Evaluation of cytotoxic and antimicrobial activity of Lysimachia thyrsiflora. 3 rd International Symposium on Natural Drugs Proceedings, Naples, 2003, 199–200.

  5. Karpova, V.I., Kintya, P.K. and Chirva, V.Y. Structure of saponin A from Naumburgia thyrsiflora. Khim. Prir. Soedin. 11 (1975) 364–366.

    CAS  Google Scholar 

  6. Kintya, P.K., Karpova, V.I. and Chirva, V.Y. Saponin from Naumburgia thyrsiflora. Khim. Prir. Soedin. 11 (1985) 520–521.

    Google Scholar 

  7. Yasukawa, K., Taira, T. and Takido, M. Flavonol gycosides from Lysimachia thyrsiflora. Biochem. Syst. Ecol. 25 (1997) 73–74.

    Article  CAS  Google Scholar 

  8. Podolak, I., Janeczko, Z., Galanty, A., Michalik, M. and Trojanowska, D. A triterpene saponin from Lysimachia thyrsiflora L. Acta Pol. Pharm. — Drug Res. 64 (2007) 39–43.

    CAS  Google Scholar 

  9. Drukała, J., Rajwa, B. and Pietrzkowski, Z. Effect of daunomycin on expression of CD44H protein in HTB140 melanoma cells in vitro. Anticancer Res. 21 (2001) 3793–3798.

    PubMed  Google Scholar 

  10. Szydłowska, H., Zaporowska, E., Kuszlik-Jochym, K., Korohoda, W. and Branny, J. Membranolytic activity of detergents as studied with cell viability tests. Folia Histochem. Cytochem. 16 (1978) 69–78.

    Google Scholar 

  11. Korohoda, W. and Madeja, Z. Contact of sarcoma cells with aligned fibroblasts accelerates their displacement, computer assisted analysis of tumour cell locomotion in co-culture. Biochem. Cell. Biol. 75 (1997) 263–276.

    Article  PubMed  CAS  Google Scholar 

  12. Friedl, P., Noble, P.B. and Zänker, K.S. Lymphocyte locomotion in three dimensional collagen gels. Comparison of three quantitative methods for analysis cell trajectories. J. Immunol. Methods 165 (1993) 157–165.

    Article  PubMed  CAS  Google Scholar 

  13. Sroka, J., Madeja, Z., Galanty, A., Michalik, M., Przestalski, S., Rakoczy, L. and Korohoda, W. Trimethyltin inhibits the chemotaxis of Dictyostelium discoideum amoebae. Europ. J. Protistol. 37 (2001) 313–326.

    Article  Google Scholar 

  14. Sroka, J., Kamiński, R., Michalik, M., Madeja, Z., Przestalski, S. and Korohoda, W. The effect of triethyllead on the motile activity of walker 256 carcinosarcoma cells. Cell. Mol. Biol. Lett. 9 (2004) 15–30.

    PubMed  CAS  Google Scholar 

  15. Seeman, P., Cheng, D. and Iles, G.H. Structure of membrane holes in osmotic and saponin hemolysis. J. Cell. Biol. 56 (1974) 519–527.

    Article  Google Scholar 

  16. Shany, S., Bernheimer, A.W., Grushoff, P.S. and Kim, K.S. Evidence for membrane cholesterol as common binding site for cereolysin, streptolysin O and saponin. Mol. Cell. Biochem. 3 (1974) 179–186.

    Article  PubMed  CAS  Google Scholar 

  17. Galeotti, T., Borello, S., Minotti, G. and Masotti, L. Membrane alternations in cancer cells: the role of oxy radicals. Ann. NY Acad. Sci. 488 (1986) 468–480.

    Article  PubMed  CAS  Google Scholar 

  18. Tugnoli, V., Trinchero, A. and Tosi, M.R. Evaluation of the lipid composition of human healthy and neoplastic renal tissues. Ital. J. Biochem. 53 (2004) 169–182.

    PubMed  CAS  Google Scholar 

  19. Pierzchalska, M., Michalik, M., Stępień, E. and Korohoda, W. Changes in morphology of human skin fibroblasts induced by local anaesthetics: role of actomyosin contraction. Eur. J. Pharm. 358 (1998) 235–244.

    Article  CAS  Google Scholar 

  20. Michalik, M., Pierzchalska, M., Pabiańczyk-Kulka, A. and Korohoda, W. Procaine-induced enhancement of fluid-phase endocytosis and inhibition of exocytosis in human skin fibroblasts. Eur. J. Pharm. 475 (2003) 1–10.

    Article  CAS  Google Scholar 

  21. Pollard, T.D. and Cooper, J.A. Actin and actin-binding proteins: a critical evaluation of mechanisms and functions. Annu. Rev. Biochem. 55 (1986) 987–1035.

    Article  PubMed  CAS  Google Scholar 

  22. Osborn, M., Born, T., Koitsch, H.J. and Weber, K. Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments. Cell 14 (1978) 477–488.

    Article  PubMed  CAS  Google Scholar 

  23. Buendia, B., Bre, M.-H. and Karsenti, E. Cytoskeletal control of centrioles movement during the establishment of polarity in Madin-Darby canine kidney cells. J. Cell. Biol. 110 (1990) 1123–1135.

    Article  PubMed  CAS  Google Scholar 

  24. Baumann, E., Stoya, G., Volkner, A., Richter, W., Lemke, C. and Linss, W. Hemolysis of human erythrocytes with saponin affects the membrane structure. Acta Histochem. 102 (2000) 21–35.

    Article  PubMed  CAS  Google Scholar 

  25. Shinkai, K., Akedo, H., Mukai, M., Imamura, F., Isoai, A., Kobayashi, M. and Kitagawa, I. Inhibition of in vitro tumor cell invasion by ginsenoside Rg3. Jpn. J. Cancer Res. 87 (1996) 357–362.

    PubMed  CAS  Google Scholar 

  26. Wakabayashi, C., Hasegawa, H., Murata, J. and Saiki, I. In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol. Res. 9 (1997) 411–417.

    PubMed  CAS  Google Scholar 

  27. Wei-Wei, Ch., Chia-Yu, Y., Tzu-Wen, L., Peng-Hui, W. and Ying-Chieh, T. Soyasaponin I decreases the expression of α2,3-linked sialic acid on the cell surface and supresses the metastatic potential of B16F10 melanoma cells. Biochem. Biophys. Res. Commun. 341 (2006) 614–619.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University, ul. Medyczna 9, 30-688, Kraków, Poland

    Agnieszka Galanty & Irma Podolak

  2. Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387, Kraków, Poland

    Marta Michalik & Łukasz Sędek

Authors
  1. Agnieszka Galanty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marta Michalik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Łukasz Sędek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Irma Podolak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Agnieszka Galanty.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Galanty, A., Michalik, M., Sędek, Ł. et al. The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells. Cell Mol Biol Lett 13, 585–598 (2008). https://doi.org/10.2478/s11658-008-0013-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2478/s11658-008-0013-x

Key words

Cellular & Molecular Biology Letters

ISSN: 1689-1392

Contact us