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Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C

Cellular & Molecular Biology Letters volume 14, pages 657–669 (2009)Cite this article

Abstract

The human SFRS9/SRp30c belongs to the SR family of splicing regulators. Despite evidence that members of this protein family may be targeted by arginine methylation, this has yet to be experimentally addressed. In this study, we found that SFRS9 is a target for PRMT1-mediated arginine methylation in vitro, and that it is immunoprecipitated from HEK-293 lysates by antibodies that recognize both mono- and dimethylated arginines. We further observed that upon treatment with the methylation inhibitor Adox, the fluorescent EGFP-SFRS9 re-localizes to dot-like structures in the cell nucleus. In subsequent confocal analyses, we found that EGFP-SFRS9 localizes to nucleoli in Adox-treated cells. Our findings indicate the importance of arginine methylation for the subnuclear localization of SFRS9.

Abbreviations

Adox:

adenosine-2′,3′-dialdehyde

Ki-1/57:

the 57-kDa protein antigen detected by the Ki-1 antibodies

mAB:

monoclonal antibodies

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Authors and Affiliations

  1. Laboratório Nacional de Luz Síncrotron, Campinas, SP, Brasil

    Gustavo C. Bressan, Eduardo C. Moraes, Tais M. Kuniyoshi, Dario O. Passos & Jörg Kobarg

  2. Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil

    Gustavo C. Bressan, Eduardo C. Moraes, Dario O. Passos & Jörg Kobarg

  3. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo, Ribeirão Preto, SP, Brasil

    Adriana O. Manfiolli & Marcelo D. Gomes

  4. Laboratório Nacional de Luz Síncrotron, Centro de Biologia Molecular Estrutural, Rua Giuseppe Máximo Scolfaro 10.000, C.P. 6192, 13084-971, Campinas, SP, Brasil

    Jörg Kobarg

Authors
  1. Gustavo C. Bressan
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  2. Eduardo C. Moraes
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  3. Adriana O. Manfiolli
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  4. Tais M. Kuniyoshi
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  5. Dario O. Passos
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  6. Marcelo D. Gomes
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  7. Jörg Kobarg
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Correspondence to Jörg Kobarg.

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Bressan, G.C., Moraes, E.C., Manfiolli, A.O. et al. Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C. Cell Mol Biol Lett 14, 657–669 (2009). https://doi.org/10.2478/s11658-009-0024-2

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  • DOI: https://doi.org/10.2478/s11658-009-0024-2

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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