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Flavonoid transport across RBE4 cells: A blood-brain barrier model

Cellular & Molecular Biology Letters volume 15, pages 234–241 (2010)Cite this article

Abstract

There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer’s and Parkinson’s diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.

Abbreviations

BBB:

blood-brain barrier

CNS:

central nervous system

GLUT1:

facilitative glucose transporter

RBE4:

rat brain endothelial cell

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Authors and Affiliations

  1. Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal

    Ana Faria, Diogo Pestana, Diana Teixeira & Conceição Calhau

  2. Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal

    Ana Faria, Joana Azevedo, Victor De Freitas & Nuno Mateus

Authors
  1. Ana Faria
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  2. Diogo Pestana
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  3. Diana Teixeira
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  4. Joana Azevedo
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  5. Victor De Freitas
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  6. Nuno Mateus
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  7. Conceição Calhau
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Correspondence to Ana Faria.

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Faria, A., Pestana, D., Teixeira, D. et al. Flavonoid transport across RBE4 cells: A blood-brain barrier model. Cell Mol Biol Lett 15, 234–241 (2010). https://doi.org/10.2478/s11658-010-0006-4

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  • DOI: https://doi.org/10.2478/s11658-010-0006-4

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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