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Integrin receptors play a role in the internalin B-dependent entry of Listeria monocytogenes into host cells

Cellular & Molecular Biology Letters volume 15, pages 496–506 (2010)Cite this article

Abstract

Listeria monocytogenes enters non-phagocytic cells by binding its surface proteins inlA (internalin) and inlB to the host’s E-cadherin and Met, respectively. The two internalins play either separate or cooperative roles in the colonization of infected tissues. Here, we studied bacterial uptake into HeLa cells using an L. monocytogenes mutant strain (ΔinlA) carrying a deletion in the gene coding for inlA. The ΔinlA mutant strain showed the capability to invade HeLa cells. The monoclonal anti-β3- and anti-β1-integrin subunit antibodies prevented bacterial uptake into the cells, while the anti-β2- and anti-β4-integrin subunit antibodies failed to affect L. monocytogenes entry into HeLa cells. Three structurally distinct disintegrins (kistrin, echistatin and flavoridin) also inhibited bacterial uptake, showing different potencies correlated to their selective affinity for the β3- and β1-integrin subunits. In addition to inducing Met phosphorylation, infection of cells by the L. monocytogenes ΔinlA mutant strain promoted the tyrosine phosphorylation of the focal adhesion-associated proteins FAK and paxillin. Our findings provide the first evidence that β3- and β1-integrin receptors play a role in the inlB-dependent internalization of L. monocytogenes into host cells.

Abbreviations

BHI:

brain-heart infusion

BSA:

bovine serum albumin

CFU:

colony-forming unit

DMEM:

Dulbecco’s modified Eagle’s medium

FAK:

focal adhesion kinase

FBS:

bovine fetal serum

InlA:

internalin A

InlB:

internalin B

MOI:

multiplicity of infection

PBS:

phosphate-buffered saline

SDS:

sodium dodecyl sulphate

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Authors and Affiliations

  1. Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Della Salute 2, 80055, Portici (Na), Italy

    Clementina Auriemma, Maurizio Viscardi, Federico Capuano & Giuseppe Iovane

  2. Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, Università degli Studi di Napoli Federico II, via F. Delpino 1, 80137, Napoli, Italy

    Simona Tafuri, Luigi Michele Pavone, Rossella Della Morte & Norma Staiano

  3. Dipartimento di Patologia e Sanità Animale, Università degli Studi di Napoli Federico II, via F. Delpino 1, 80137, Napoli, Italy

    Laura Rinaldi & Giuseppe Iovane

Authors
  1. Clementina Auriemma
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  2. Maurizio Viscardi
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  4. Luigi Michele Pavone
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  9. Norma Staiano
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Correspondence to Norma Staiano.

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Auriemma, C., Viscardi, M., Tafuri, S. et al. Integrin receptors play a role in the internalin B-dependent entry of Listeria monocytogenes into host cells. Cell Mol Biol Lett 15, 496–506 (2010). https://doi.org/10.2478/s11658-010-0019-z

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  • DOI: https://doi.org/10.2478/s11658-010-0019-z

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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