Open Access

TRPC expression in mesenchymal stem cells

  • Frederic Torossian1, 2,
  • Aurelie Bisson1,
  • Jean-Pierre Vannier2,
  • Olivier Boyer1 and
  • Marek Lamacz1, 3Email author
Cellular & Molecular Biology LettersAn International Journal201015:31

DOI: 10.2478/s11658-010-0031-3

Received: 23 March 2010

Accepted: 23 August 2010

Published: 28 August 2010

Abstract

Transient receptor potential canonical (TRPC) channels are key players in calcium homeostasis and various regulatory processes in cell biology. Little is currently known about the TRPC subfamily members in mesenchymal stem cells (MSC), where they could play a role in cell proliferation. We report on the presence of TRPC1, 2, 4 and 6 mRNAs in MSC. Western blot and immunofluorescence staining indicate a membrane and intracellular distribution of TRPC1. Furthermore, the decrease in the level of TRPC1 protein caused by RNA interference is accompanied by the downregulation of cell proliferation. These results indicate that MSC express TRPC1, 2, 4 and 6 mRNA and that TRPC1 may play a role in stem cell proliferation.

Key words

Mesenchymal stem cells TRPC1 Cell proliferation

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