Open Access

Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity

  • Xuejiao Zhang1,
  • Cheng Li1,
  • Huiling Gao1,
  • Hiroaki Nabeka1,
  • Tetsuya Shimokawa1,
  • Hiroyuki Wakisaka1,
  • Seiji Matsuda1 and
  • Naoto Kobayashi2Email author
Cellular & Molecular Biology LettersAn International Journal201116:6

DOI: 10.2478/s11658-011-0006-z

Received: 22 October 2010

Accepted: 2 March 2011

Published: 9 March 2011

Abstract

We investigated the effects of Rho-associated kinase (ROCK) on migration and cytoskeletal organization in primary human osteoblasts and Saos-2 human osteosarcoma cells. Both cell types were exposed to two different ROCK inhibitors, Y-27632 and HA-1077. In the improved motility assay used in the present study, Y-27632 and HA-1077 significantly increased the migration of both osteoblasts and osteosarcoma cells on plastic in a dose-dependent and reversible manner. Fluorescent images showed that cells of both types cultured with Y-27632 or HA-1077 exhibited a stellate appearance, with poor assembly of stress fibers and focal contacts. Western blotting showed that ROCK inhibitors reduced myosin light chain (MLC) phosphorylation within 5 min without affecting overall myosin light-chain protein levels. Inhibition of ROCK activity is thought to enhance the migration of human osteoblasts through reorganization of the actin cytoskeleton and regulation of myosin activity. ROCK inhibitors may be potentially useful as anabolic agents to enhance the biocompatibility of bone and joint prostheses.

Key words

Osteoblast Migration ROCK inhibitor Cytoskeleton Stress fiber Focal contact MLC phosphorylation

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