Open Access

Developmental expression of P5 ATPase mRNA in the mouse

  • Lisa S. Weingarten1,
  • Hardi Dave2,
  • Hongyan Li2 and
  • Dorota A. Crawford1, 2, 3Email author
Cellular & Molecular Biology LettersAn International Journal201117:39

DOI: 10.2478/s11658-011-0039-3

Received: 11 July 2011

Accepted: 15 December 2011

Published: 28 December 2011

Abstract

P5 ATPases (ATP13A1 through ATP13A5) are found in all eukaryotes. They are currently poorly characterized and have unknown substrate specificity. Recent evidence has linked two P5 ATPases to diseases of the nervous system, suggesting possible importance of these proteins within the nervous system. In this study we determined the relative expression of mouse P5 ATPases in development using quantitative real time PCR. We have shown that ATP13A1 and ATP13A2 were both expressed similarly during development, with the highest expression levels at the peak of neurogenesis. ATP13A3 was expressed highly during organogenesis with one of its isoforms playing a more predominant role during the period of neuronal development. ATP13A5 was expressed most highly in the adult mouse brain. We also assessed the expression of these genes in various regions of the adult mouse brain. ATP13A1 to ATP13A4 were expressed differentially in the cerebral cortex, hippocampus, brainstem and cerebellum while levels of ATP13A5 were fairly constant between these brain regions. Moreover, we demonstrated expression of the ATP13A4 protein in the corresponding brain regions using immunohistochemistry. In summary, this study furthers our knowledge of P5-type ATPases and their potentially important role in the nervous system.

Key words

P5-type ATPases mRNA expression Neurogenesis Parkinson’s disease Autism spectrum disorders Real-time PCR Immunohistochemistry

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