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Protective effect of placenta extracts against nitrite-induced oxidative stress in human erythrocytes

Cellular & Molecular Biology Letters volume 17, pages 240–248 (2012)Cite this article

Abstract

Aqueous-saline human placenta extract (HPE) is known to possess antioxidant activity due to the high concentration of bioactive substances. This fact allows its application in clinical practice in order to treat oxidation-induced diseases. Extract antioxidant activity is mainly conditioned by proteins. Freezing of extracts has been shown to lead to their antioxidant activity increasing due to protein conformation changes.

Different biological models are widely used in order to evaluate efficacy of novel antioxidants and mechanisms of their action. One such model appears to be erythrocytes under nitrite-induced oxidative stress. Nitrite is known to be able to penetrate erythrocyte membrane and to oxidize hemoglobin. In order to investigate whether HPE is able to decrease nitrite-induced oxidative injuries and to evaluate the protein contribution to this process, spectrophotometric and electron spin resonance (ESR) assays were used.

Experimental data have revealed that antioxidant activity of extracts and of some of their fractions correlates with methemoglobin concentration lowering. Preliminary erythrocyte incubation with an extract fraction of 12 kDa allows preservation of the structural-dynamic cytosol state the closest to the control.

Abbreviations

ESR:

electron spin resonance

ABTS:

2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)

HPE:

human placenta extract

RBC:

red blood cell

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Authors and Affiliations

  1. Department of Cryobiophysics, Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine, Kharkiv, Ukraine

    Svitlana Rozanova, Yana Cherkashina, Svitlana Repina, Katherina Rozanova & Oleg Nardid

Authors
  1. Svitlana Rozanova
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  2. Yana Cherkashina
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  3. Svitlana Repina
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  4. Katherina Rozanova
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  5. Oleg Nardid
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Corresponding author

Correspondence to Svitlana Rozanova.

Additional information

Paper authored by participant of the international conference: 18th Meeting, European Association for Red Cell Research, Wrocław — Piechowice, Poland, May 12–15th, 2011. Publication cost was covered by the organizers of this meeting.

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Rozanova, S., Cherkashina, Y., Repina, S. et al. Protective effect of placenta extracts against nitrite-induced oxidative stress in human erythrocytes. Cell Mol Biol Lett 17, 240–248 (2012). https://doi.org/10.2478/s11658-012-0007-6

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  • DOI: https://doi.org/10.2478/s11658-012-0007-6

Key words

Cellular & Molecular Biology Letters

ISSN: 1689-1392

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