Open Access

Analysis of the role of the integrin signaling pathway in hepatocytes during rat liver regeneration

  • Cunshuan Xu1, 2Email author,
  • Yanjie Yang1, 2,
  • Junying Yang2,
  • Xiaoguang Chen2 and
  • Gaiping Wang2
Cellular & Molecular Biology LettersAn International Journal201217:11

DOI: 10.2478/s11658-012-0011-x

Received: 25 August 2011

Accepted: 22 February 2012

Published: 7 March 2012

Abstract

To explore the role of the integrin signaling pathway in hepatocytes during rat liver regeneration, the integrin signaling pathway-related gene expression profile in hepatocytes of regenerative liver was detected using Rat Genome 230 2.0 array. The chip data showed that 265 genes of the integrin signaling pathway were included by Rat Genome 230 2.0 array and 132 genes showed significant expression changes in hepatocytes of regenerative liver. The numbers of up-, down- and up/down-regulated genes were 110, 15 and 7 respectively. In addition, bioinformatics and systems biology methods were used to analyze the role of the integrin signaling pathway in hepatocytes. The analysis of gene synergy value indicated that paths 1, 8, 12, and 15 promoted hepatocyte proliferation at the priming phase of liver regeneration; paths 1, 3, 8, and 12–15 enhanced hepatocyte proliferation at the progressing phase; paths 11 and 14 promoted hepatocyte proliferation, while paths 12 and 13 reduced hepatocyte proliferation at the terminal phase. Additionally, the other 8 paths (2, 4, 5–7, 9–10, and 16) were not found to be related to liver regeneration. In conclusion, 132 genes and 8 cascades of the integrin signaling pathway participated in regulating hepatocyte proliferation during rat liver regeneration.

Key words

Integrin signaling pathway Rat Liver regeneration Partial hepatectomy Immunochemistry Hepatocyte proliferation Rat Genome 230 2.0 array RT-PCR Gene expression profile Gene synergy value

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