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Modulation of physiological and pathological activities of lysozyme by biological membranes

Cellular & Molecular Biology Letters volume 17, pages 349–375 (2012)Cite this article

Abstract

The molecular details of interactions between lipid membranes and lysozyme (Lz), a small polycationic protein with a wide range of biological activities, have long been the focus of numerous studies. The biological consequences of this process are considered to embrace at least two aspects: i) correlation between antimicrobial and membranotropic properties of this protein, and ii) lipid-mediated Lz amyloidogenesis. The mechanisms underlying the lipid-assisted protein fibrillogenesis and membrane disruption exerted by Lz in bacterial cells are believed to be similar. The present investigation was undertaken to gain further insight into Lz-lipid interactions and explore the routes by which Lz exerts its antimicrobial and amyloidogenic actions. Binding and Förster resonance energy transfer studies revealed that upon increasing the content of anionic lipids in lipid vesicles, Lz forms aggregates in a membrane environment. Total internal reflection fluorescence microscopy and pyrene excimerization reaction were employed to study the effect of Lz on the structural and dynamic properties of lipid bilayers. It was found that Lz induces lipid demixing and reduction of bilayer free volume, the magnitude of this effect being much more pronounced for oligomeric protein.

Abbreviations

CD:

circular dichroism

CL:

cardiolipin

DTT:

dithiothreitol

DyL549 :

DyLight 549

E/M:

pyrene excimer-to-monomer intensity ratio

ESR:

electron spin resonance

Fl:

fluorescein

FRET:

Förster resonance energy transfer

G+:

Gram positive bacteria

G−:

Gram-negative bacteria

HLH:

helix-loop-helix domain

IR:

infrared

LPS:

lipopolysaccharide

LUV:

large unilamellar vesicles

Lz:

lysozyme

PBS:

phosphate buffered saline

PIE:

pulse interleaved excitation

PC:

phosphatidylcholine

PG:

phosphatidylglycerol

PS:

phosphatidylserine

SeTau647 :

SeTau-647-di-NHS

SLB:

supported lipid bilayer

TIRFM:

total internal reflection fluorescence microscopy

tr-FRET:

time-resolved FRET

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Authors and Affiliations

  1. Department of Biological and Medical Physics, V. N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077, Ukraine

    Valeriya Trusova

  2. 19-32 Geroyev Truda St., Kharkov, 61144, Ukraine

    Valeriya Trusova

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  1. Valeriya Trusova
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Trusova, V. Modulation of physiological and pathological activities of lysozyme by biological membranes. Cell Mol Biol Lett 17, 349–375 (2012). https://doi.org/10.2478/s11658-012-0015-6

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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