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Phylogenetic origin and transcriptional regulation at the post-diauxic phase of SPI1, in Saccharomyces cerevisiae

Cellular & Molecular Biology Letters volume 17, pages 393–407 (2012)Cite this article

Abstract

The gene SPI1, of Saccharomyces cerevisiae, encodes a cell wall protein that is induced in several stress conditions, particularly in the postdiauxic and stationary phases of growth. It has a paralogue, SED1, which shows some common features in expression regulation and in the null mutant phenotype. In this work we have identified homologues in other species of yeasts and filamentous fungi, and we have also elucidated some aspects of the origin of SPI1, by duplication and diversification of SED1. In terms of regulation, we have found that the expression in the post-diauxic phase is regulated by genes related to the PKA pathway and stress response (MSN2/4, YAK1, POP2, SOK2, PHD1, and PHO84) and by genes involved in the PKC pathway (WSC2, PKC1, and MPK1).

Abbreviations

AP:

adaptor protein

C:

control

GPI:

glycophosphatidylinositol

mRNA:

messenger RNA

OD:

optical density

te]ONPG:

ortho-nitrophenyl-β-galactoside

PD:

post-diauxic

PKA:

protein kinase A

PKC:

protein kinase C

S:

SD without glucose

SD:

synthetic defined medium

YPD:

yeast extract peptone dextrose medium

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Authors and Affiliations

  1. Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Paterna, Spain

    Fernando Cardona & Agustín Aranda

  2. Departament de Bioquímica i Biologia Molecular, Universitat de València, Burjassot, Spain

    Fernando Cardona & Marcel.Lí Del Olmo

Authors
  1. Fernando Cardona
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  2. Marcel.Lí Del Olmo
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  3. Agustín Aranda
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Correspondence to Fernando Cardona.

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Cardona, F., Del Olmo, M. & Aranda, A. Phylogenetic origin and transcriptional regulation at the post-diauxic phase of SPI1, in Saccharomyces cerevisiae . Cell Mol Biol Lett 17, 393–407 (2012). https://doi.org/10.2478/s11658-012-0017-4

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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