Fig. 3

NF-kB and STAT3 inhibitors alter SKOV-3 cell viability and affect Wnt5A and ROR2 expression. a – MTT assay of cell viability in the presence of BAY11-7082 as an NF-kB inhibitor (5, 10 μM) or S31-201 as a STAT3 inhibitor (25, 50 μM). b – Normalized values (means ± SD) from three independent western blots for Wnt5A in the presence of BAY11-7082 for the indicated times. c – Normalized values (means ± SD) from three independent western blots for Wnt5A in the presence of S31-201 for the indicated times. D – Western blot analysis of Wnt5A in cells treated with BAY11-7082 (5, 10 μM) or S31-201 (25, 50 μM) for the indicated times. The western blots represent one of three independent experiments. E – Normalized values (means ± SD) from three independent western blots for ROR2 in the presence of BAY11-7082 or S31-201 for 24 and 48 h. F- Western blot analysis of ROR2 in cells treated with BAY11-7082 (5 μM) or S31-201 (25 μM) for 24 and 48 h. The western blots represent one of three independent experiments. GAPDH levels were used as the internal control. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 as compared with the control (Ctrl)