Fig. 6

Wnt5A is involved in rhIL-6-induced migration in SKOV-3 cells. a – Western blot quantification showed a 70 % reduction in Wnt5A expression in siRNA Wnt5A-transfected cells compared with the controls. The western blots represent one of three independent experiments. GAPDH levels were used as the internal control. b – In vitro cell migration assay of non-transfected or siRNA Wnt5A-transfected cells in the absence or presence of rhIL-6. The upper panel showed photos of transwells in the indicated conditions and the lower panel showed quantification of migrated cells in ten random fields. The percentage of migrated cells was expressed as: (number of migrated cells/number of seeded cells × 100). The migration index was expressed relative to control cells (set as 100 %). All of the experiments were carried out three times and the results are expressed as means ± SD. Magnification: 100x. *p ≤ 0.05; **p ≤ 0.0 as compared with the control (Ctrl)