Type | MDSC-P | MDSC-D1 | MDSC-D3 |
|---|
Count | % | Count | % | Count | % |
|---|
total_reads | 6000000 | Â | 6000000 | Â | 6000000 | Â |
high_quality | 5984373 | 100Â % | 5984335 | 100Â % | 5984569 | 100Â % |
3'adaptor_null | 2916 | 0.05Â % | 2967 | 0.05Â % | 2901 | 0.05Â % |
insert_null | 3115 | 0.05Â % | 1252 | 0.02Â % | 1408 | 0.02Â % |
5'adaptor_contaminants | 86464 | 1.44Â % | 54566 | 0.91Â % | 39651 | 0.66Â % |
smaller_than_18nt | 20713 | 0.35Â % | 3297 | 0.06Â % | 4615 | 0.08Â % |
polyA | 110 | 0.00Â % | 65 | 0.00Â % | 31 | 0.00Â % |
clean_reads | 5871055 | 98.11Â % | 5922188 | 98.96Â % | 5935963 | 99.19Â % |
- Note: total_reads: total sequenced reads, which is required to be greater than 5Â M in general; high_quality: number of high quality reads with no N, no more than 4 bases whose quality score was lower than 10 and no more than 6 bases whose quality score was lower than 13; 3'adaptor_null: number of reads with no 3'adaptor; insert_null: number of reads with no insertion; 5'adaptor_contaminants: number of 5'contaminants; smaller_than_18nt: number of reads less than 18Â nt generally. Small RNA tags were between 18 and 30Â nt long, so too short tags should be removed from data for further analysis; polyA: number of reads with polyA; clean_reads: number of clean reads after adaptors and contaminants were removed, which were used in the following analysis
