|
Type
|
MDSC-P
|
MDSC-D1
|
MDSC-D3
|
|---|
|
Count
|
%
|
Count
|
%
|
Count
|
%
|
|---|
|
total_reads
|
6000000
| |
6000000
| |
6000000
| |
|
high_quality
|
5984373
|
100 %
|
5984335
|
100 %
|
5984569
|
100 %
|
|
3'adaptor_null
|
2916
|
0.05 %
|
2967
|
0.05 %
|
2901
|
0.05 %
|
|
insert_null
|
3115
|
0.05 %
|
1252
|
0.02 %
|
1408
|
0.02 %
|
|
5'adaptor_contaminants
|
86464
|
1.44 %
|
54566
|
0.91 %
|
39651
|
0.66 %
|
|
smaller_than_18nt
|
20713
|
0.35 %
|
3297
|
0.06 %
|
4615
|
0.08 %
|
|
polyA
|
110
|
0.00 %
|
65
|
0.00 %
|
31
|
0.00 %
|
|
clean_reads
|
5871055
|
98.11 %
|
5922188
|
98.96 %
|
5935963
|
99.19 %
|
- Note: total_reads: total sequenced reads, which is required to be greater than 5 M in general; high_quality: number of high quality reads with no N, no more than 4 bases whose quality score was lower than 10 and no more than 6 bases whose quality score was lower than 13; 3'adaptor_null: number of reads with no 3'adaptor; insert_null: number of reads with no insertion; 5'adaptor_contaminants: number of 5'contaminants; smaller_than_18nt: number of reads less than 18 nt generally. Small RNA tags were between 18 and 30 nt long, so too short tags should be removed from data for further analysis; polyA: number of reads with polyA; clean_reads: number of clean reads after adaptors and contaminants were removed, which were used in the following analysis
