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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Effects of NH4CL application and removal on astrocytes and endothelial cells

Fig. 3

NH4Cl addition and removal stimulates [Ca2+]i changes in astrocytes. a and b – Fluorescence images, acquired using an excitation wavelength of 380 nm, of a group of astrocytes loaded with Fura-2/AM. A – Astrocytes at the beginning of the experiment. b – The same cells after being exposed to NH4Cl. The morphology of the cells remained unchanged. c – An example of average F340/F380 as a function of time in astrocyte cell culture (n = 7). T1 – time point before the substitution of the SBS with the NH4Cl bathing solution; T2 – time point at which the maximum change of F340/F380 was reached after the substitution of the SBS with the NH4Cl bathing solution; T3 – time point (at 900 s) before substituting the NH4Cl bathing solution with the SBS; T4 – time point of the maximum change of F340/F380 after substituting the NH4Cl bathing solution with the SBS

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