Fig. 6

NH₄Cl addition and removal stimulates [Ca²⁺]_i changes in endothelial cells. a and b – Fluorescence images, acquired using an excitation wavelength of 380 nm, of a group of ECs loaded with Fura-2/AM. A – ECs at the beginning of the experiment. b – The same cells after being exposed to NH₄Cl. The morphology of the cells remained unchanged. c – An example of average F₃₄₀/F₃₈₀ as a function of time in astrocyte cell culture (n = 18). T1 – time point before the substitution of the SBS with the NH₄Cl bathing solution; T2 – time point at which the maximum change of F₃₄₀/F₃₈₀ was reached after the substitution of the SBS with the NH₄Cl bathing solution; T3 – time point (at 900 s) before substituting the NH₄Cl bathing solution with the SBS; T4 – time point of the maximum change of F₃₄₀/F₃₈₀ after substituting the NH₄Cl bathing solution with the SBS; d – Changes after NH₄Cl addition plotted as trends. e – Changes after NH₄Cl removal plotted as trends. Boxplots on each side present median, upper and lower quartile, minimum and maximum and outliers. Experiments are numbered using consecutive numbers as performed