Fig. 1

Hypoxia induces dynamic changes in the mRNA expression profiles of the NOS3, HIF1A and HIF2A (EPAS1) in human human umbilical vein endothelial cells (HUVECs). The mRNA levels during hypoxia were monitored in qRT-PCR experiments as described in [49, 50]. The results from 2 independent experiments (n = 8) are plotted normalized to 18S rRNA levels and expressed as a fold-change compared to the normoxic control. The hypoxia had no impact on HUVECs viability as monitored with Real Time xCelligence as described in [106]. Error bars represent standard deviations. Significant changes (p < 0.05) are marked with an “*”