Fig. 5From: Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneoProteins differentially expressed by HTR-8/SVneo cells cultured under serum-depleted conditions. a HTR-8/SVneo cells were grown in medium with 10 or 0Ā % FBS for 24Ā h (in triplicate). Total protein extracts (0.50Ā mg) were separated by 18 cm 2DE gels as previously described (nā=ā3). The protein spots were analyzed via LC ESI-Q/TOF and identified using bioinformatics analysis. MW ā molecular weight marker (RPN5800, GE). b Effect of serum depletion on the expression of some trophoblast proteins. Statistical analysis of 2DE protein spots identified by MS/MS in HTR-8/SVneo proteomes either with 10Ā % FBS or without FBS (nā=ā3). Kolmogorov-Smirnov test (D value): 0.10, Dā>ā0.642 (a, Dā=ā1.0; b, Dā=ā0.67)Back to article page