Fig. 7From: Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneoVimentin western blot analysis. HTR-8/SVneo cells were grown in medium with 10 or 0Ā % FBS for 24Ā h. Total protein extracts (30Ā Ī¼g) of independent experimental triplicates were separated by SDS-PAGE. Western blot, as previously described, was used to analyze the protein levels of vimentin. a Representative western blot films obtained. MW ā molecular weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Scientific). b Densitometric quantifications of expected vimentin band (54Ā kDa) were made in triplicate to each film using Quantity One software. FBS 10Ā % ND ā not detectableBack to article page