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Table 1 Relevant proteins identified via MS/MS and bioinformatics analysis for the effects of serum depletion

From: Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneo

Gene name Protein name MW (Da) pI~ MS/MS score % Seq Pep match K test Fold FBS 0%/10% Molecular function Biological process
ACTG1 Actin, cytoplasmic 2 41793 5.31 221.9 47 15 1.00 a Structural constituent of cytoskeleton Cellular component movement
VCP Transitional endoplasmic reticulum ATPase 89322 5.14 350.8 39 25 1.00 a Hydrolase DNA repair, vesicle transport
ANXA1 Annexin A1 38714 6.64 98.6 17 48 1.00 a Helicase activity, Receptor binding Cell surface receptor signaling pathway
HSP90AA1 Heat shock protein HSP 90-alpha 84660 4.94 37.67 4 3 1.00 a Chaperone Stress response
HSPA8 Heat shock cognate 71 kDa protein 70898 5.37 562.7 68 36 1.00 -3.14 Chaperone, repressor Stress response, transcription
HNRNPL Heterogeneous nuclear ribonucleoprotein L 64133   47.04 6 4 1.00 -2.75 Transcription regulatory region DNA binding. RNA processing
KRT8 Keratin, type II cytoskeletal 8 53705 5.52 261.4 41 21 1.00 -1.51 Structural constituent of cytoskeleton, structural molecule activity MET, cytoskeleton organization
HSPD1 60 kDa heat shock protein, mitochondrial 61055 5.7 497.2 66 34 0.67 -1.35 Chaperone Host–virus interaction
VIM Vimentin 53652 5.06 332.7 47 23 0.67 1.51 Structural constituent of cytoskeleton EMT
DLD Dihydrolipoyl dehydrogenase, mitochondrial 54150 6.92 54.93 9 4 1.00 1.66 Dihydrolipoyl dehydrogenase activity Cell redox homeostasis, lysine catabolic process
PKM2 Pyruvate kinase isozymes M1/M2 57937 7.96 329.1 44 22 1.00 1.94 Kinase, transferase Glycolysis
IMPDH2 Inosine-5’-monophosphate dehydrogenase 2 55805 6.44 83.48 13 6 0.67 2.08 Oxidoreductase GMP and purine biosynthesis
ENO1 Alpha-enolase 47169 6.99 142.5 26 11 0.67 2.07 Lyase, repressor Glycolysis, transcription regulation
  1. Protein spots were analyzed via LC-ESI-MS-Q/TOF and identified using the MASCOT search engine. MW (Da) – theoretical molecular weight in Dalton; pI ~ – theoretical pI; MS/MS score – protein score given by Mascot; % Seq – percentage sequence coverage; Pep match – number of peptides assigned to protein; K test – D value from Kolmogorov-Smirnov test (0.10, D > 0.642); Fold FBS 0%/10 % – fold optical density of the protein spot (expression) for serum depleted proteomes (0 % FBS) compared to control proteomes (10 % FBS); a – uniquely expressed protein spot. Relevant GO terms for molecular function and biological process are used
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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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