Skip to main content

Table 1 Relevant proteins identified via MS/MS and bioinformatics analysis for the effects of serum depletion

From: Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneo

Gene name

Protein name

MW (Da)

pI~

MS/MS score

% Seq

Pep match

K test

Fold FBS 0%/10%

Molecular function

Biological process

ACTG1

Actin, cytoplasmic 2

41793

5.31

221.9

47

15

1.00

a

Structural constituent of cytoskeleton

Cellular component movement

VCP

Transitional endoplasmic reticulum ATPase

89322

5.14

350.8

39

25

1.00

a

Hydrolase

DNA repair, vesicle transport

ANXA1

Annexin A1

38714

6.64

98.6

17

48

1.00

a

Helicase activity, Receptor binding

Cell surface receptor signaling pathway

HSP90AA1

Heat shock protein HSP 90-alpha

84660

4.94

37.67

4

3

1.00

a

Chaperone

Stress response

HSPA8

Heat shock cognate 71 kDa protein

70898

5.37

562.7

68

36

1.00

-3.14

Chaperone, repressor

Stress response, transcription

HNRNPL

Heterogeneous nuclear ribonucleoprotein L

64133

Ā 

47.04

6

4

1.00

-2.75

Transcription regulatory region DNA binding.

RNA processing

KRT8

Keratin, type II cytoskeletal 8

53705

5.52

261.4

41

21

1.00

-1.51

Structural constituent of cytoskeleton, structural molecule activity

MET, cytoskeleton organization

HSPD1

60 kDa heat shock protein, mitochondrial

61055

5.7

497.2

66

34

0.67

-1.35

Chaperone

Hostā€“virus interaction

VIM

Vimentin

53652

5.06

332.7

47

23

0.67

1.51

Structural constituent of cytoskeleton

EMT

DLD

Dihydrolipoyl dehydrogenase, mitochondrial

54150

6.92

54.93

9

4

1.00

1.66

Dihydrolipoyl dehydrogenase activity

Cell redox homeostasis, lysine catabolic process

PKM2

Pyruvate kinase isozymes M1/M2

57937

7.96

329.1

44

22

1.00

1.94

Kinase, transferase

Glycolysis

IMPDH2

Inosine-5ā€™-monophosphate dehydrogenase 2

55805

6.44

83.48

13

6

0.67

2.08

Oxidoreductase

GMP and purine biosynthesis

ENO1

Alpha-enolase

47169

6.99

142.5

26

11

0.67

2.07

Lyase, repressor

Glycolysis, transcription regulation

  1. Protein spots were analyzed via LC-ESI-MS-Q/TOF and identified using the MASCOT search engine. MW (Da) ā€“ theoretical molecular weight in Dalton; pIā€‰~ā€‰ā€“ theoretical pI; MS/MS score ā€“ protein score given by Mascot; % Seq ā€“ percentage sequence coverage; Pep match ā€“ number of peptides assigned to protein; K test ā€“ D value from Kolmogorov-Smirnov test (0.10, Dā€‰>ā€‰0.642); Fold FBS 0%/10Ā % ā€“ fold optical density of the protein spot (expression) for serum depleted proteomes (0Ā % FBS) compared to control proteomes (10Ā % FBS); a ā€“ uniquely expressed protein spot. Relevant GO terms for molecular function and biological process are used