Fig. 2

Interaction between Exo70 and the cytoskeleton in A7r5 cells. a α-actin and Exo70 co-localization in A7r5 cells. Immunofluorescent detection of α-actin (green fluorescent) and Exo70 (red fluorescence) expressions. The yellow area is a α-actin and Exo70 merged visualization, indicating their co-localization. Scale length is 75 μm. b Tubulin and Exo70 co-localization in A7r5 cells. Immunofluorescent detection of tubulin (green fluorescent) and Exo70 (red fluorescence) expressions. The blue fluorescence is showing the nuclei stained with DAPI. The yellow area indicating tubulin and Exo70 expression overlap is not present, suggesting the absence of co-localization. Scale length is 75 μm. c α-actin and Exo70 co-localization in A7r5 cells after 1 h treatment with cytochalasin B. Immunofluorescent detection of α-actin (green fluorescent) and Exo70 (red fluorescence) in A7r5 cells, the blue fluorescence is showing the nuclei stained with DAPI. The image on the left shows that α-Actin, Exo70, and the nucleus overlap, suggesting that α-actin depolymerization has occurred. Scale length is 100 μm