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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: The role of Exo70 in vascular smooth muscle cell migration

Fig. 4

Exo70knockdown inhibits A7r5 cell migration and invasion. a Wound healing assay in different A7r5 cell lines. Images of the cells were taken at 0, 24, 40 and 48 h after the wound healing assay, magnification of 200 times. b Transwell assays of A7r5 cell migration. Images of the cells were taken and cells were counted 24 h after being seeded in the chambers. n = 3, three independent replicates. c Quantification of wound healing assay. Images of the A7r5 cells were taken every 8 h, and the time needed to reach a complete wound closure was determined. n = 3. Three independent clones of stable cell lines were tested. *p < 0.05; ***p < 0.001. d Quantification of migrating cells through transwell assay. The bars indicate the average number of migrating cells per field for each group. n = 10. *p < 0.05; ns, not statistically significant

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