Fig. 3
From: The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner
Specificity test of AKR7A2 and CYGB interaction in Y2HGold. Y2HGold cells were co-transformed with Empty pGBKT7 + Empty pGADT7, pGBKT7-CYGB + Empty pGADT7, Empty pGBKT7 + pGADT7-AKR7A2 or pGBKT7-CYGB + pGADT7-AKR7A2 (images as indicated). Positive control: Y2HGold cells transformed with pGBKT7-53 + pGADT7-T. Negative control: Y2HGold cells transformed with pGBKT7-lam + pGADT7-T. The positive interaction between AKR7A2 and CYGB was confirmed by the growth and blue color on SD/-Leu/-Trp/-His/-Ade/X-α-gal/AbA agar plates