Fig. 4
From: The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner
Confirmation of the interaction between AKR7A2 and CYGB in HEK 293Â T cells. a The expression of FLAG-tagged CYGB protein in HEK 293Â T cells. b The expression of MYC-tagged AKR7A2 protein in HEK 293Â T cells. c Co-immunoprecipitation of AKR7A2 and CYGB. HEK293T cells were co-transfected using pCMV-MYC-AKR7A2 together with pcDNA3.0-FLAG or pcDNA3.0-FLAG-CYGB vectors and then subjected to immunoprecipitation with ANTI-FLAG M2 Affinity Gel, followed by immunoblotting with anti-MYC and anti-FLAG antibody