Table 1 Primers used in this study
From: The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner
| Constructs | Primer sequence (5′-3′) |
|---|---|
| pcDNA3.0-FLAG-CYGB | F: AGCTTAGATTACAAGGATGACGACGATAAGATGGAGAAAG |
| R: CGGAATTCCTACGGCCCCGAAGAGG | |
| pGBKT7-CYGB | F: CGGAATTCATGGAGACAGGCGAG |
| R: AAAACTGCAGCTACGGCCCCGAAGAGG | |
| pCMV-MYC-AKR7A2 | F: CGAATTCAAATGCTGAGTGCCGCGTCT |
| R: CCGACTCGAGCTAGCGGAAGTAGTTGGGA | |
| pGADT7-AKR7A2 | F: CGAATTCATGCTGAGTGCCGCGTCT |
| R: CCGACTCGAGCTAGCGGAAGTAGTTGGGA |