Table 1 Primers used in this study
From: The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner
Constructs | Primer sequence (5′-3′) |
|---|---|
pcDNA3.0-FLAG-CYGB | F: AGCTTAGATTACAAGGATGACGACGATAAGATGGAGAAAG |
| Â | R: CGGAATTCCTACGGCCCCGAAGAGG |
pGBKT7-CYGB | F: CGGAATTCATGGAGACAGGCGAG |
| Â | R: AAAACTGCAGCTACGGCCCCGAAGAGG |
pCMV-MYC-AKR7A2 | F: CGAATTCAAATGCTGAGTGCCGCGTCT |
| Â | R: CCGACTCGAGCTAGCGGAAGTAGTTGGGA |
pGADT7-AKR7A2 | F: CGAATTCATGCTGAGTGCCGCGTCT |
| Â | R: CCGACTCGAGCTAGCGGAAGTAGTTGGGA |