Fig. 3
From: The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro
Expression of osteoblast markers, i.e., type I collagen (COLI), alkaline phosphatase (ALP), osteocalcin (OC) determined using semi-quantitative RT-PCR. Analysis of the representative gels and the level of GAPDH, COLI, ALP and OC mRNA (the average optical density of bands; Odu) is presented in the charts (n = 3). Results are normalized to the housekeeping gene level (GAPDH). 0 – cell cultured under undifferentiating conditions in standard medium; 7, 14, 21 – cells cultured under osteogenic conditions in differentiating medium