Table 1 List of primers used in the RT-PCR analysis
From: The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro
Target gene | Primer sequences | Fragment size (bp) |
|---|---|---|
ALP | F 5’-ACGTGGCTAAGAATGTCATC-3’ R 5’-AGGGCTCCAACGAGATCGAGATCCG-3’ | 475 |
COLI | F 5’-AACTGAAAGCTGAATCCTTCCA-3’ R 5-’TGCCCAAGAAACAAAGCTTC-3’ | 223 |
GAPDH | F 5’-TCAAGGAAGCTACGGGCA-3’ R 5’-TGGCAGAAATTACACACACACAC-3’ | 250 |
β1 integrin subunit | F 5-’AAGGGCGTGTTGGTAGACATT-3’ R 5’-TGACACATCTCACACGTTTGC-3’ | 424 |