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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: IgG silencing induces apoptosis and suppresses proliferation, migration and invasion in LNCaP prostate cancer cells

Fig. 1

IGHG1 silencing suppresses the clonogenicity and survival of LNCaP cells. Cells were transfected with IgG1 (siIgG) and control siRNA (siCon). a – Cells were harvested at 36 h for qPCR to determine the mRNA expression levels. b – Cells were harvested at 48 h for western blotting to determine the protein expression levels. c – Transfected cells were used for a colony-forming assay. Colonies were stained with crystal violet and then counted to calculate the colony-forming efficiency (CFE). d – The same stained colonies were counted to determine the surviving fraction (SF). *p < 0.01 vs. siCon; # p < 0.01 vs. LNCaP (non-transfected control)

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