Fig. 1From: IgG silencing induces apoptosis and suppresses proliferation, migration and invasion in LNCaP prostate cancer cellsIGHG1 silencing suppresses the clonogenicity and survival of LNCaP cells. Cells were transfected with IgG1 (siIgG) and control siRNA (siCon). a ā Cells were harvested at 36Ā h for qPCR to determine the mRNA expression levels. b ā Cells were harvested at 48Ā h for western blotting to determine the protein expression levels. c ā Transfected cells were used for a colony-forming assay. Colonies were stained with crystal violet and then counted to calculate the colony-forming efficiency (CFE). d ā The same stained colonies were counted to determine the surviving fraction (SF). *pā<ā0.01 vs. siCon; # pā<ā0.01 vs. LNCaP (non-transfected control)Back to article page