Fig. 3

IGHG1 silencing reduces in vitro migration and invasion of LNCaP cells. Cells were transfected with IgG1 (siIgG) and control siRNA (siCon) and then seeded into transwells to determine a – The cell migration ability and b – The cell invasion ability. For migration assays, the cells were seeded in inserts containing fresh medium. For invasion assays, the inserts contained matrigel. Representative pictures are shown (scale bar, 100 μM). c, d – Quantitative results from three independent experiments are shown. *p < 0.05 vs. siCon; ^# p < 0.05 vs. LNCaP (non-transfected control)