Fig. 4

IGHG1 silencing decreases PCNA and increases caspase-3 in LNCaP cells. Cells were transfected with IgG1 (siIgG) and control siRNA (siCon) for 48 h and then fixed with 4% paraformaldehyde and stained for a – PCNA proliferation marker and b – caspase-3 apoptotic marker. Representative images are shown (scale bar, 100 μM). c, d – The integrated optical density (IOD) of green fluorescence and the area were quantified. *p < 0.05 vs. siCon; ^# p < 0.05 vs. LNCaP (non-transfected control)